Differentiation of Pyrus Genotypes
The Challenges of Morphological Identification
Secondly, the poor morphological characteristics and diversity of Pyrus further complicate the process of identification. Finally, the widespread cross-ability and interspecific hybridization, and introgression among different species make it even more difficult to differentiate between different genotypes.
The Emergence of Molecular Techniques
Given the limitations of morphological identification, researchers have turned to molecular techniques for a better understanding of Pyrus genotypes. The recent application of molecular biology and tool has revolutionized due to the use of DNA for a better understanding of genome structure, evolution, and identification of species. Marker techniques are used by prototypes of pear genetic maps, such as amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR), And random amplified polymorphic DNA (RAPD). In particular, SSR markers have emerged as a key tool in the differentiation of Pyrus genotypes.
SSR Markers: A Common Tool for Differentiation
In constructing pear genetic maps, among different types of markers, SSR markers have been widely used because of their co-dominant and transferable nature. This can be used as common markers to compare and construct consensus maps. From 2007, various Pear genetic maps were constructed based on SSR markers. These maps have helped to shed light on the genetic relationships between different Pyrus genotypes.
Conclusion
In conclusion, the differentiation of Pyrus genotypes is a challenging task, given the limitations of morphological identification. However, molecular techniques such as SSR markers have emerged as key tools for differentiation. These techniques have helped researchers to build a more comprehensive understanding of the genetic relationships between different Pyrus genotypes. As further research is conducted, we can expect to see even more sophisticated approaches to the differentiation and identification of Pyrus genotypes.
1.1: Homology tree of pyrus:
1.2:DNA marker used for Pyrus species
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- 4: SSR marker used in pear:
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