DNA ISOLATION IN PEAR

: Pyrus pic

 DNA Isolation in Pear

Pears are known for their delicious taste and unique texture. However, identifying and analyzing different Pyrus species has proven to be difficult due to low morphological variety and overlapping characteristics. 

Importance of DNA Disconnection from Pears

Prior to beginning the point-by-point biosystematics of pears, updating the convention for its DNA disconnection from pears was essential. DNA isolation is essential for most molecular biological applications like PCR. However, when it comes to DNA confinement of mature trees of high elevation like pears, it becomes a challenging task due to the presence of numerous polyphenolic compounds and polysaccharides. Different protocols have been developed for the isolation of high-yielding quality DNA, but the long processing time, the need for costly supplies, expensive kits, chemicals, and low-quality yield are some of the factors that hinder their application in fruit trees.

Identification of Pyrus Species

The isolation and analysis of Pyrus species can be difficult due to low morphological variety and overlapping characteristics. To overcome this problem, the utilization of DNA has revolutionized the identification and study of genome design, evolution, and species identification.

Challenges in DNA Isolation

The bad quality yield is due to an assortment of chemical constituents in trees, which needs to be modified in the convention properly. DNA degradation is mediated by secondary plant products, such as phenolic terpenoids that bind to DNA after lysis support, making it difficult to isolate good-quality DNA from plants containing a high concentration of polyphenolics such as pears.

DNA Isolation Protocols

DNA isolation of pears was developed by Saghai-Maroof et al., where the isolation of cores was derived from the extended, costly, and low-yielding cesium chloride-ethidium bromide ultracentrifugation method that produced only corrupted DNA from soybean leaves. Doyle and Doyle applied the same method as Saghai Maroof et al. with some modifications, which worked better. The method was published at various times by different researchers. One such modification used a changed CTAB for the extraction of high-quality DNA, which is suitable for SSR. In this method, high salt concentration was used for the removal of polysaccharides and PVP for polyphenolic compounds, making it suitable for the extraction of high-quality DNA.

Pear Production and Trade

In 2005-2006, world pear exports increased by 6% and reached 1.6 million tons, despite a slight decrease in pear exports from Argentina and China. On the Northern Hemisphere, exports increased by 12%, supported by growth in most revealing countries. Trade volumes from the US increased by 6% with a lower production level, while China witnessed an increase in pear production due to establishing areas and advanced tree management. China produces more than other countries of the world, having an annual production of 8 million tons, and the area under cultivation is 0.94 million hectares. The prominent cultivars are mainly produced from Pyrus Bret Schneider Rehd., P. ussuriensis Adage, P. serotina Rehd, and P. sinkiangensis, and many are imported from Japan.

Conclusion

In conclusion, DNA isolation from pears is crucial for the identification and analysis of Pyrus species. Despite the challenges posed by the presence of numerous chemical constituents, various protocols have been developed to extract high-quality DNA. The growth in pear production and trade globally highlights the importance of DNA isolation in pears for the study of genome design, evolution, and species identification.